Coding

Part:BBa_K654060:Design

Designed by: Cody Tramp   Group: iGEM11_Utah_State   (2011-09-26)


Fatty Acyl-CoA Reductase with 8-His Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1709
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 659
    Illegal NgoMIV site found at 802
    Illegal AgeI site found at 25
    Illegal AgeI site found at 1424
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mutagenesis removal of PstI site, without altering amino acid sequence


Source

Marinobacter aquaeolei genomic DNA

References